Publications

Zhang, Qingrong ; Rosa, Raissa S. L. ; Ray, Ankita ; Durlet, Kimberley ; Dorrazehi, Gol Mohammad ; Bernardi, Rafael C. ; Alsteens, David.

Probing SARS-CoV-2 membrane binding peptide via single-molecule AFM-based force spectroscopy. In: Nature Communications, Vol. 16, no.1, p. 6 (2025). doi:10.1038/s41467-024-55358-9.

Shang, Pengcheng ; dos Santos Natividade, Rita ; Taylor, Gwen M. ; Ray, Ankita ; Welsh, Olivia L. ; Fiske, Kay L. ; Sutherland, Danica M. ; Alsteens, David ; Dermody, Terence S..

NRP1 is a receptor for mammalian orthoreovirus engaged by distinct capsid subunits. In: Cell Host & Microbe, Vol. 32, no.6, p. 980-995.e9 (2024). doi:10.1016/j.chom.2024.04.014 (Accepté/Sous presse).

Stommen, Amaury ; Ghodsi, Marine ; Cloos, Anne-Sophie ; Conrard, Louise ; Dumitru, Andra-Cristina ; Henriet, Patrick ; Pierreux, Christophe ; Alsteens, David ; Tyteca, Donatienne.

Piezo1 Regulation Involves Lipid Domains and the Cytoskeleton and Is Favored by the Stomatocyte–Discocyte–Echinocyte Transformation. In: Biomolecules, Vol. 14, no.1, p. 51 [1-27] (2024). doi:10.3390/biom14010051.

Alsteens, David.

Probing living cell dynamics and molecular interactions using atomic force microscopy. In: Biophysical Reviews, (2024). doi:10.1007/s12551-024-01258-7 (Accepté/Sous presse).

Ray, Ankita ; Minh Tran, Thu Thi ; Santos Natividade, Rita dos ; Moreira, Rodrigo A. ; Simpson, Joshua D. ; Mohammed, Danahe ; Koehler, Melanie ; Petitjean, Simon J.L. ; Zhang, Qingrong ; Bureau, Fabrice ; Gillet, Laurent ; Poma, Adolfo B. ; Alsteens, David.

Single-Molecule Investigation of the Binding Interface Stability of SARS-CoV-2 Variants with ACE2. In: ACS Nanoscience Au, Vol. 4, no.2, p. 136-145 (2024). doi:10.1021/acsnanoscienceau.3c00060 (Accepté/Sous presse).

dos Santos Natividade, Rita ; Dumitru, Andra-Cristina (co-first author) ; Nicoli, Alessandro ; Strebl, Michael ; Sutherland, Danica M. ; Welsh, Olivia L. ; Ghulam, Mustafa ; Stehle, Thilo ; Dermody, Terence S. ; Di Pizio, Antonella ; Koehler, Melanie ; Alsteens, David.

Viral capsid structural assembly governs the reovirus binding interface to NgR1. In: Nanoscale Horizons, Vol. 9, no.11, p. 1925-1937 (2024). doi:10.1039/d4nh00315b.

Fernandez, Maricruz ; Shkumatov, Alexander V ; Liu, Yun ; Stulemeijer, Claire ; Derclaye, Sylvie ; Efremov, Rouslan G ; Hallet, Bernard ; Alsteens, David.

AFM-based force spectroscopy unravels stepwise formation of the DNA transposition complex in the widespread Tn3 family mobile genetic elements.. In: Nucleic acids research, Vol. 51, no.10, p. 4929-4941 (2023). doi:10.1093/nar/gkad241.

Vanderroost, Juliette ; Avalosse, Noémie ; Mohammed, Danahe ; Hoffmann, Delia ; Henriet, Patrick ; Pierreux, Christophe ; Alsteens, David ; Tyteca, Donatienne.

Cholesterol and sphingomyelin polarize at the leading edge of migrating myoblasts and involve their clustering in submicrometric domains.. In: Biomolecules, Vol. 13, no. 2, p. 319 [1-30] (2023). doi:10.3390/biom13020319.

Dos Santos Natividade, Rita ; Koehler, Melanie ; Gomes, Priscila S F C ; Simpson, Joshua ; Smith, Sydni Caet ; Gomes, Diego E B ; de Lhoneux, Juliette ; Yang, Jinsung ; Ray, Ankita ; Dermody, Terence S ; Bernardi, Rafael C ; Ogden, Kristen M ; Alsteens, David.

Deciphering molecular mechanisms stabilizing the reovirus-binding complex.. In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 120, no.21, p. e2220741120 (2023). doi:10.1073/pnas.2220741120.

Petitjean, Simon ; Eeckhout, Savannah ; Delguste, Martin ; Zhang, Qingrong ; Durlet, Kimberley ; Alsteens, David.

Heparin-Induced Allosteric Changes in SARS-CoV-2 Spike Protein Facilitate ACE2 Binding and Viral Entry.. In: Nano letters, Vol. 23, no.24, p. 11678-11684 (2023). doi:10.1021/acs.nanolett.3c03550.

Glorieux, Laura ; Vandooren, Laura ; Derclaye, Sylvie ; Pyr dit Ruys, Sébastien ; Oncina-Gil, Paloma ; Salowka, Anna ; Herinckx, Gaëtan ; Aajja Maazoubi, Elias ; Lemoine, Pascale ; Spourquet, Catherine ; Lefort, Hélène ; Henriet, Patrick ; Tyteca, Donatienne ; Spagnoli, Francesca M. ; Alsteens, David ; Vertommen, Didier ; Pierreux, Christophe.

In-Depth Analysis of the Pancreatic Extracellular Matrix during Development for Next-Generation Tissue Engineering. In: International Journal of Molecular Sciences, Vol. 24, no.12, p. 10268 [1-22] (2023). doi:10.3390/ijms241210268.

Sutherland, Danica M ; Strebl, Michael ; Koehler, Melanie ; Welsh, Olivia L ; Yu, Xinzhe ; Hu, Liya ; Dos Santos Natividade, Rita ; Knowlton, Jonathan J ; Taylor, Gwen M ; Moreno, Rodolfo A ; Wörz, Patrick ; Lonergan, Zachery R ; Aravamudhan, Pavithra ; Guzman-Cardozo, Camila ; Kour, Sukhleen ; Pandey, Udai Bhan ; Alsteens, David ; Wang, Zhao ; Prasad, B V Venkataram ; Stehle, Thilo ; Dermody, Terence S.

NgR1 binding to reovirus reveals an unusual bivalent interaction and a new viral attachment protein.. In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 120, no.24, p. e2219404120 (2023). doi:10.1073/pnas.2219404120.

Shang, Pengcheng ; Simpson, Joshua ; Taylor, Gwen M ; Sutherland, Danica M ; Welsh, Olivia L ; Aravamudhan, Pavithra ; Dos Santos Natividade, Rita ; Schwab, Kristina ; Michel, Joshua J ; Poholek, Amanda C ; Wu, Yijen ; Rajasundaram, Dhivyaa ; Koehler, Melanie ; Alsteens, David ; Dermody, Terence S.

Paired immunoglobulin-like receptor B is an entry receptor for mammalian orthoreovirus.. In: Nature communications, Vol. 14, no.1, p. 2615 (2023). doi:10.1038/s41467-023-38327-6.

Ledoux, Benjamin ; Zanin, Natacha ; Yang, Jinsung ; Mercier, Vincent ; Coster, Charlotte ; Dupont-Gillain, Christine C. ; Alsteens, David ; Renard, Henri-François.

Plasma membrane nanodeformations promote actin polymerization through CIP4/CDC42 recruitment and regulate type II IFN signaling, collab. Morsomme, Pierre. In: Science Advances, Vol. 9, no.50, p. 1 (2023). doi:10.1126/sciadv.ade1660.

Simpson, Joshua ; Ray, Ankita ; Marcon, Claire ; Dos Santos Natividade, Rita ; Dorrazehi, Gol Mohammad ; Durlet, Kimberley ; Koehler, Melanie ; Alsteens, David.

Single-Molecule Analysis of SARS-CoV-2 Binding to C-Type Lectin Receptors.. In: Nano letters, Vol. 23, no.4, p. 1496-1504 (2023). doi:10.1021/acs.nanolett.2c04931.

Simpson, Joshua D. ; Ray, Ankita ; Koehler, Melanie ; Mohammed, Danahe ; Alsteens, David.

Atomic force microscopy applied to interrogate nanoscale cellular chemistry and supramolecular bond dynamics for biomedical applications. In: Chemical Communications, Vol. 58, no.33, p. 5072-5087 (2022). doi:10.1039/d1cc07200e (Soumis).

Petitjean, Simon J. L. ; Chen, Wenzhang ; Koehler, Melanie ; Jimmidi, Ravikumar ; Yang, Jinsung ; Mohammed, Danahe ; Juniku, Blinera ; Stanifer, Megan L. ; Boulant, Steeve ; Vincent, Stéphane P. ; Alsteens, David.

Multivalent 9-O-Acetylated-sialic acid glycoclusters as potent inhibitors for SARS-CoV-2 infection. In: Nature Communications, Vol. 13, no.1, p. 2564 (2022). doi:10.1038/s41467-022-30313-8.

Lo Giudice, Maria ; Yang, Jinsung ; Poncin, Mégane A. ; Adumeau, Laurent ; Delguste, Martin ; Koehler, Melanie ; Evers, Koen ; Dumitru, Andra-Cristina ; Dawson, Kenneth A. ; Alsteens, David.

Nanophysical Mapping of Inflammasome Activation by Nanoparticles via Specific Cell Surface Recognition Events. In: ACS Nano, Vol. 16, no.1, p. 306-316 (2021). doi:10.1021/acsnano.1c06301 (Accepté/Sous presse).

Petitjean, Simon ; Lecocq, Marylène ; Lelong, Camille ; Denis, Robin ; Defrère, Sylvie ; Mariage, Pierre-Antoine ; Alsteens, David ; Pilette, Charles.

Salvia miltiorrhiza Bunge as a Potential Natural Compound against COVID-19. In: Cells, Vol. 11, no. 8, p. 1311 [1-12] (2022). doi:10.3390/cells11081311.

Zhang, Qingrong ; Yang, Jinsung ; Tillieux, Sueli ; Guo, Zhengyuan ; Natividade, Rita dos Santos ; Koehler, Melanie ; Petitjean, Simon ; Cui, Zongqiang ; Alsteens, David.

Stepwise Enzymatic-Dependent Mechanism of Ebola Virus Binding to Cell Surface Receptors Monitored by AFM. In: Nano Letters, Vol. 22, no.4, p. 1641-1648 (2022). doi:10.1021/acs.nanolett.1c04677 (Accepté/Sous presse).

Maja, Mauriane ; Mohammed, Danahe ; Dumitru, Andra-Cristina ; Verstraeten, Sandrine ; Lingurski, Maxime ; Mingeot-Leclercq, Marie-Paule ; Alsteens, David ; Tyteca, Donatienne.

Surface cholesterol-enriched domains specifically promote invasion of breast cancer cell lines by controlling invadopodia and extracellular matrix degradation. In: Cellular and molecular life sciences, Vol. 79, no. 8, p. 417 [1-20] (2022). doi:10.1007/s00018-022-04426-8.

Mohammed, Danahe ; Koehler, Melanie ; Dumitru, Andra-Cristina ; Aravamudhan, Pavithra ; Sutherland, Danica M. ; Dermody, Terence S. ; Alsteens, David.

Altered Glycan Expression on Breast Cancer Cells Facilitates Infection by T3 Seroptype Oncolytic Reovirus. In: Nano Letters, Vol. 21, no.22, p. 9720-9728 (2021). doi:10.1021/acs.nanolett.1c03608.

Müller, Daniel J. ; Dumitru, Andra-Cristina ; Lo Giudice, Maria ; Gaub, Hermann E. ; Hinterdorfer, Peter ; Hummer, Gerhard ; De Yoreo, James J. ; Dufrêne, Yves ; Alsteens, David.

Atomic Force Microscopy-Based Force Spectroscopy and Multiparametric Imaging of Biomolecular and Cellular Systems. In: Chemical Reviews, no. 121, p. 11701-11725 (2020). doi:10.1021/acs.chemrev.0c00617.

Yang, Jinsung ; Petitjean, Simon ; Koehler, Mélanie ; Zhang, Qingrong ; Dumitru, Andra-Cristina ; Chen, Wenzhang ; Derclaye, Sylvie ; Vincent, Stéphane P ; Soumillion, Patrice ; Alsteens, David.

Author Correction: Molecular interaction and inhibition of SARS-CoV-2 binding to the ACE2 receptor.. In: Nature communications, Vol. 12, no.1, p. 2996 (2021). doi:10.1038/s41467-021-23498-x.

Koehler, Mélanie ; Petitjean, Simon ; Yang, Jinsung ; Aravamudhan, Pavithra ; Somoulay, Xayathed ; Lo Giudice, Maria ; Poncin, Mégane ; Dumitru, Andra-Cristina ; Dermody, Terence S ; Alsteens, David.

Author Correction: Reovirus directly engages integrin to recruit clathrin for entry into host cells.. In: Nature communications, Vol. 12, no.1, p. 2946 (2021). doi:10.1038/s41467-021-23537-7.

Viljoen, Albertus ; Mathelie-Guinlet, Marion ; Ray, Ankita ; Strohmeyer, Nico ; Oh, Yoo Jin ; Hinterdorfer, Peter ; Müller, Daniel J. ; Alsteens, David ; Dufrêne, Yves.

Force spectroscopy of single cells using atomic force microscopy. In: Nature Reviews Methods Primers, Vol. 1, no.1, p. 63 (2021). doi:10.1038/s43586-021-00062-x.

Cloos, Anne-Sophie ; Daenen, Laura G. M. ; Maja, Mauriane ; Stommen, Amaury ; Vanderroost, Juliette ; Van Der Smissen, Patrick ; Rab, Minke ; Westerink, Jan ; Mignolet, Eric ; Larondelle, Yvan ; Terrasi, Romano ; Muccioli, Giulio ; Dumitru, Andra-Cristina ; Alsteens, David ; van Wijk, Richard ; Tyteca, Donatienne.

Impaired Cytoskeletal and Membrane Biophysical Properties of Acanthocytes in Hypobetalipoproteinemia – A Case Study. In: Frontiers in Physiology, Vol. 12, no. 2, p. 638027 [1-22] (2021). doi:10.3389/fphys.2021.638027.

Pakravan, Donya ; Michiels, Emiel ; Bratek-Skicki, Anna ; De Decker, Mathias ; Van Lindt, Joris ; Alsteens, David ; Derclaye, Sylvie ; Van Damme, Philip ; Schymkowitz, Joost ; Rousseau, Frederic ; Tompa, Peter ; Van Den Bosch, Ludo.

Liquid–Liquid Phase Separation Enhances TDP-43 LCD Aggregation but Delays Seeded Aggregation. In: Biomolecules, Vol. 11, no.4, p. 548 (2021). doi:10.3390/biom11040548 (Soumis).

Koehler, Melanie ; Ray, Ankita ; Moreira, Rodrigo A. ; Juniku, Blinera ; Poma, Adolfo B. ; Alsteens, David.

Molecular insights into receptor binding energetics and neutralization of SARS-CoV-2 variants. In: Nature Communications, Vol. 12, no.1 (2021). doi:10.1038/s41467-021-27325-1.

Dumitru, Andra-Cristina ; Stommen, Amaury ; Koehler, Melanie ; Cloos, Anne-Sophie ; Yang, Jinsung ; Leclercqz, Arnaud ; Tyteca, Donatienne ; Alsteens, David.

Probing PIEZO1 Localization upon Activation Using High-Resolution Atomic Force and Confocal Microscopy. In: Nano Letters, Vol. 21, no.12, p. 4950-4958 (2021). doi:10.1021/acs.nanolett.1c00599.

Koehler, Mélanie ; Petitjean, Simon ; Yang, Jinsung ; Aravamudhan, Pavithra ; Somoulay, Xayathed ; Lo Giudice, Maria ; Poncin, Mégane ; Dumitru, Andra-Cristina ; Dermody, Terence S. ; Alsteens, David.

Reovirus directly engages integrin to recruit clathrin for entry into host cells. In: Nature Communications, Vol. 12, no.1, p. 1-15 (2021). doi:10.1038/s41467-021-22380-0.

Poncin, Mégane A. ; Van Meerbeeck, Pierre ; Simpson, Joshua D. ; Clippe, André ; Tyckaert, François ; Bouillenne, Fabrice ; Degand, Hervé ; Matagne, André ; Morsomme, Pierre ; Knoops, Bernard ; Alsteens, David.

Role of the Redox State of Human Peroxiredoxin-5 on Its TLR4-Activating DAMP Function. In: Antioxidants, Vol. 10, no.12, p. 1902 (2021). doi:10.3390/antiox10121902.

Yang, Jinsung ; Park, Jeongsoo ; Koehler, Melanie ; Simpson, Joshua ; Luque, Daniel ; Rodríguez, Javier M. ; Alsteens, David.

Rotavirus Binding to Cell Surface Receptors Directly Recruiting α2 Integrin. In: Advanced NanoBiomed Research, Vol. NaN, no. Nan, p. 2100077 (2021). doi:10.1002/anbr.202100077.

Delguste, Martin ; Le Brun, Grégoire ; Cotin, Florian ; Machiels, Bénédicte ; Gillet, Laurent ; Alsteens, David.

Single-Virus Force Spectroscopy Discriminates the Intrinsic Role of Two Viral Glycoproteins upon Cell Surface Attachment. In: Nano Letters, Vol. 21, no. 1, p. 847-853 (2021). doi:10.1021/acs.nanolett.0c04609.

Mathelie-Guinlet, Marion ; Viela Bovio, Felipe ; Alsteens, David ; Dufrêne, Yves.

Stress-Induced Catch-Bonds to Enhance Bacterial Adhesion. In: Trends in Microbiology, Vol. 1, no.1, p. 1 (2020). doi:10.1016/j.tim.2020.11.009.

Koehler, Melanie ; Lo Giudice, Cristina ; Vogl, Philipp ; Ebner, Andreas ; Hinterdorfer, Peter ; Gruber, Hermann J. ; Alsteens, David.

Control of Ligand-Binding Specificity Using Photocleavable Linkers in AFM Force Spectroscopy. In: Nano Letters, Vol. 20, no.5, p. 4038-4042 (2020). doi:10.1021/acs.nanolett.0c01426.

Renard, Henri-François ; Tyckaert, François ; Lo Giudice, Maria ; Hirsch, Thibault ; Valades-Cruz, Cesar Augusto ; Lemaigre, Camille ; Shafaq-Zadah, Massiullah ; Wunder, Christian ; Wattiez, Ruddy ; Johannes, Ludger ; van der Bruggen, Pierre ; Alsteens, David ; Morsomme, Pierre.

Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166. In: Nature Communications, Vol. 11, no.1, p. 1457 (2020). doi:10.1038/s41467-020-15303-y.

Mathelie-Guinlet, Marion ; Viela Bovio, Felipe ; Pietrocola, Giampiero ; Speziale, Pietro ; Alsteens, David ; Dufrêne, Yves.

Force-clamp spectroscopy identifies a catch bond mechanism in a Gram-positive pathogen. In: Nature Communications, Vol. 11, no.1, p. 1 (2020). doi:10.1038/s41467-020-19216-8.

Köhler, Melanie ; Delguste, Martin ; Sieben, Christian ; Gillet, Laurent ; Alsteens, David.

Initial step of virus entry: virion binding to cell surface glycans. In: Annual Review of Virology, (2020) (Accepté/Sous presse).

Dumitru, Andra-Cristina ; Mohammed, Danahe ; Maja, Mauriane ; Yang, Jinsung ; Verstraeten, Sandrine ; del Campo, Aranzazu ; Mingeot-Leclercq, Marie-Paule ; Tyteca, Donatienne ; Alsteens, David.

Label‐Free Imaging of Cholesterol Assemblies Reveals Hidden Nanomechanics of Breast Cancer Cells. In: Advanced Science, p. 2002643 (2020). doi:10.1002/advs.202002643.

Viljoen, Albertus ; Alsteens, David ; Dufrêne, Yves.

Mechanical Forces between Mycobacterial Antigen 85 Complex and Fibronectin. In: Cells, Vol. 9, no.3, p. 716 (2020). doi:10.3390/cells9030716.

Lo Giudice, Cristina ; Zhang, Haonan ; Wu, Beili ; Alsteens, David.

Mechanochemical Activation of Class-B G-Protein-Coupled Receptor upon Peptide–Ligand Binding. In: Nano Letters, Vol. 20, no.7, p. 5575-5582 (2020). doi:10.1021/acs.nanolett.0c02333.

Yang, Jinsung ; Petitjean, Simon ; Koehler, Mélanie ; Zhang, Qingrong ; Dumitru, Andra-Cristina ; Chen, Wenzhang ; Derclaye, Sylvie ; Vincent, Stéphane P. ; Soumillion, Patrice ; Alsteens, David.

Molecular interaction and inhibition of SARS-CoV-2 binding to the ACE2 receptor. In: Nature Communications, Vol. 11, no. 1, p. 4541 (2020). doi:10.1038/s41467-020-18319-6.

Valotteau, Claire ; Dumitru, Andra-Cristina ; Lecordier, Laurence ; Alsteens, David ; Pays, Etienne ; Pérez-Morga, David ; Dufrêne, Yves.

Multiparametric Atomic Force Microscopy Identifies Multiple Structural and Physical Heterogeneities on the Surface of Trypanosoma brucei. In: ACS Omega, Vol. 5, no.33, p. 20953-20959 (2020). doi:10.1021/acsomega.0c02416.

Diller, Julia R ; Halloran, Sean R ; Koehler, Melanie ; Dos Santos Natividade, Rita ; Alsteens, David ; Stehle, Thilo ; Dermody, Terence S ; Ogden, Kristen M.

Reovirus σ1 Conformational Flexibility Modulates the Efficiency of Host Cell Attachment.. In: Journal of virology, Vol. 94, no.23, p. e01163-20 [1-18] (2020). doi:10.1128/JVI.01163-20.

Dumitru, Andra-Cristina ; Deepak, R. N. V. Krishna ; Liu, Heng ; Koehler, Melanie ; Zhang, Cheng ; Fan, Hao ; Alsteens, David.

Submolecular probing of the complement C5a receptor–ligand binding reveals a cooperative two-site binding mechanism. In: Communications Biology, Vol. 3, no.1, p. 786 (2020). doi:10.1038/s42003-020-01518-8.

Lecordier, Laurence ; Uzureau, Sophie ; Vanwalleghem, Gilles ; Deleu, Magali ; Crowet, Jean-Marc ; Barry, Paul ; Moran, Barry ; Voorheis, Paul ; Dumitru, Andra-Cristina ; Yamaryo-Botté, Yoshiki ; Dieu, Marc ; Tebabi, Patricia ; Vanhollebeke, Benoit ; Lins, Laurence ; Botté, Cyrille Y. ; Alsteens, David ; Dufrêne, Yves ; Pérez-Morga, David ; Nolan, Derek P. ; Pays, Etienne.

The Trypanosoma Brucei KIFC1 Kinesin Ensures the Fast Antibody Clearance Required for Parasite Infectivity. In: iScience, Vol. 23, no.9, p. 101476 (2020). doi:10.1016/j.isci.2020.101476 (Accepté/Sous presse).