Transgenesis Platform (TRSG)
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Picture credit : Pixabay - Gerd Altmann
Technical and scientific expertise
Support for the design and creation of transgenic mouse lines
- Application of national and European ethical regulations
- Generation and use of mouse lines subject to prior approval by the concerned university ethics committee
- Creation of new mouse lines using:
- injection of DNA into fertilised oocytes
- injection of genetically modified embryonic stem cells into blastocysts
- implementation of the state-of-the-art CRISPR-Cas9 technology
- Creation of conditional knock-out mice and targeted mutations as well as insertion of knock-in cassettes
- Cryopreservation and cryopreparation of mouse sperm
- Transfer of sterile embryos.
Main equipement
- Semi-enclosed facility in compliance with strict health regulations as defined by Jackson laboratories
- Sterilised equipment, food and drink
- Pathogen testing in accordance with the recommendations of the Federation of European Laboratory Animal Science Associations (FELASA)
Applications
- Generation of CRISPR-Cas9 mouse models
- Generation of transgenic mice by injection of DNA structures into fertilised oocytes
- Homologous recombination
- Revitalisation of transgenic lines from frozen sperm and vitrified embryos
Services available to
- UCLouvain students and researchers
- UCLouvain national and international partner university researchers